TwinsensorBT

(ß-lactam + Tetracycline detection)

Features of Twinsensor

TwinsensorBT is a receptor-based assay in a dipstick format allowing rapid and simultaneous detection of ß-lactams and tetracyclines present in a milk sample.
TwinsensorBT is a competitive test involving two specific receptors in one single operation.
TwinsensorBT is rapid and takes only 6 minutes to run at 50°C ± 3°C.
TwinsensorBT is easy to read by comparing the colour intensities of the lines.
TwinsensorBT is appropriate to make testing in situ - farm, collecting centres, dairies, milk processing industries and even analytical laboratories.

Twinsensor dipstick

There are 3 capture lines on the dipstick. Two (2) test lines located on both sides (top and below) of one (1) central control line.

The control line is weak and visible all the time. The colour intensity of the control line serves as a threshold for comparing test lines intensities.
The below test line is the specific line for penicillin and cephalosporin compounds. Remember Below for Betalactams.
The top test line is the specific line for tetracycline compounds. Remember Top for Tetracyclines.

Test procedure

Add milk (200µl) to the reagent microwell and homogenise with the pipette.
Place the microwell onto the heater block for 3 min.
Add dipstick to microwell and incubate it for another 3 min.
Take out the dipstick and interpret the colour intensities.
Record the result with Quantisensor (optional).

Reading the results

Comparing the intensity of the «TEST» lines with that of the «CTRL» line allows a visual interpretation of the results.

When the «TEST» lines - either bottom ß-lactam or top tetracycline - are more visible than the «CTRL» line, the sample is considered to be NEGATIVE and contains a lower antibiotic concentration than the Detection Limits.
When the «TEST» lines are as visible as the «CTRL» line or less visible than the «CTRL» line, the sample is considered to be POSITIVE and contains an antibiotic concentration that is equal to or higher than the Detection Limits.
No «TEST» line indicates a HIGHLY POSITIVE sample for both ß-lactam and Tetracycline compounds.

When hesitating, you should regard the sample as positive and confirm by having a second interpretation four (4) minutes later.

Detection limits

Coumpounds	Twinsensor BT	MRL
Ampicillin	3 - 5	4
Amoxicillin	3 - 6	4
Benzylpenicillin	2 - 3	4
Cefazolin	20 - 25	50
Cefoperazone	2 - 3	50
Ceftiofur	10 - 15	100
Cephapirin	4 - 8	60
Cloxacillin	4 - 8	30
Nafcillin	40 - 50	30
Chlortetracycline	25 - 30	100
Doxycycline	10 - 20	100
Oxytetracycline	30 - 40	100
Tetracycline	40 - 50	100

Kit format

TwinsensorBT is available in kits of 96 tests (code BT00640) or 24 tests (code BT00641).

BT00640 contains everything to perform 96 measurements :

12 strips of 8-reagent microwells in a zip-grip aluminium pouch with desiccant
4 bottles with 24 dipsticks
1 empty tray for micro-wells
1 minipet of 200 µl
96 disposable tips for the minipet
1 information notice

BT00641 contains everything to perform 24 measurements :

3 bottles with 1 strip of 8-reagent micro-wells
1 bottle of with 24 dipsticks
1 empty tray for micro-wells
1 minipet of 200 µl
24 disposable tips for the minipet
1 information notice

Equipment required

Dry Block heater "Heatsensor" (code HS00642) is available from Unisensor. A water bath at 50°C ± 3°C can be used.
Dipstick reader "Quantisensor" (code QS00497) is available from Unisensor.

BT
ß-lactam
+ Tetracycline